This presents a challenge for system builders who want to efficiently build and use purpose-made instruments for conducting scientific experiments.
As this challenge was relevant for all of the projects described in this thesis, it was the first one to be tackled by the development of the software package Instrumentino.
This thesis focuses on instrumental advances in microfluidics-based capillary electrophoresis systems for achieving various goals.
The RNAscope was also found to be independent of the HIV-1 subtype and can be applied to patient samples at single cell level.
In paper IV, a microﬂuidic chip was developed to simultaneously isolate these three mononuclear leukocyte cell types directly from whole blood.
The microﬂuidic method reduces the sample volume requirement and is a promising tool for latent HIV-1 reservoir study. Morshed, Kajsa Noyan, Aman Russom, Anders Sönnerborg, Ujjwal Neogi.
These proteins might have the potential to be used as surface biomarkers for latent HIV-1 reservoir.
CD4 T cells, monocyte/macrophages, and natural killer cells are believed to be the primary source for HIV-1 reservoirs in peripheral blood.
Several protein catalysts have been effectively immobilized on the surface of commercial and microfabricated electrodes by electrochemically assisted deposition in sol-gel and poly-(o-phenylenediamine) polymer matrices and characterised with confirmed catalytic activity.